Solidagenone, a labdane diterpene isolated from inflorescences of Solidago chilensis Meyen, Asteraceae, was investigated for its anti-inflammatory and anti-asthmatic action. Allergic pleurisy and allergic lung inflammation assays were performed at doses of 1, 10, 50, and 100 mg/kg in ovalbumin-sensitized mice. In vitro cytotoxicity was performed, using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] MTT assay in a murine J774A1 macrophage cell line and human blood cells at concentrations of 0.1–100 µg/ml, as well as genotoxicity using the comet assay. In addition, in silico predictions were performed using ADMET Predictor TM software to predict absorption, distribution, metabolism, excretion, and toxicity. This study showed the anti-inflammatory activity and the possibility of an anti-asthmatic action of solidagenone at a concentration of 6 mg considering calculations for adult humans. Furthermore, it was possible to assess toxicity in vitro under the conditions performed and compare it with the in silico predictions.

By Deise Cristina Drummond Xavier Paes Lopes, Thais Morais de Brito, Fausto Klabund Ferraris, Taline Ramos Conde, Thayná Rocco Machado, Denise de Abreu Garófalo, Alessandra Mendonça Teles de Souza, Bárbara Abrahim-Vieira, Temistocles Barroso Oliveira, Ralph Santos-Oliveira, Alessandra Lifsitch Viçosa, Simone Sacramento Valverde & Eduardo Ricci Junio