The aim of this tutorial is to develop a population PK model for a multiple dosing trial study of a hypothetical test drug used in the treatment of diabetes. This case study is part of the Pharmacometrics web-based learning resource from the center for translational medicine of the University of Maryland (https://ctm.umaryland.edu/#/ms-pharma… workflow consists of the preparation of a data set, choosing a PK model, estimating parameters and analyzing goodness of fits and results. Datxplore, Monolix and Sycomore are used.